en Microbiology Microbiology Original Article Original Article <span style="font-size:14px;"><span style="font-family:times new roman;"><span style="color:#0000FF;"><strong><em>Background</em></strong><em>:</em></span> Torque teno virus (TTV) is a non-enveloped DNA virus that its role as a helper or causative agent in hepatitis is still unclear. TTV prevalence varies in different regions of the world. This study aimed to determine the prevalence of TTV in healthy individuals and those infected with hepatitis C virus (HCV) living in Yazd city, Iran.<br> <span style="color:#0000FF;"><strong><em>Methods</em></strong><em>:</em></span> In this case-control study, 50 healthy subjects and 68 HCV-positive individuals who referred to Yazd hospitals participated in this study. TTV DNA in serum samples were detected by nested polymerase chain reaction (PCR) using specific primers of 5΄-UTR and N22 regions. The genotypes of HCV and TTV were determined by sequencing method.<br> <span style="color:#0000FF;"><strong><em>Results</em></strong><em>:</em></span> TTV-DNA was detected in 2 out of 50 (4℅) healthy individuals and in 4 out of 68 (5.8℅) HCV-positive persons. There was not a significant correlation between the prevalence of TTV and HCV infection. The most common TTV genotypes among HCV-positive individuals were 3, 17 and 13, respectively. There was not a significant association obtained between HCV genotypes and TTV genotypes.<br> <span style="color:#0000FF;"><strong><em>Conclusion</em></strong><em>:</em> </span>The prevalence of TTV in Yazd province was low compared with the other areas of Iran. The prevalence of TTV in HCV infected people was not significantly higher than its rate in uninfected individuals.</span></span> Genotype, Hepatitis C, Prevalence, Torque teno virus 199 204 http://caspjim.com/browse.php?a_code=A-10-1109-3&slc_lang=en&sid=1 Mansour Moghimi moghimi@yahoo.com 100319475328460021772 100319475328460021772 No Department of Pathology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Mohammad Shayestehpour shayesteh@gmail.com 100319475328460021773 100319475328460021773 No Autoimmune Diseases Research Center, Kashan University of Medical Sciences, Kashan, I.R. Iran Masoud Doosti doosti@yahoo.com 100319475328460021774 100319475328460021774 No Infectious and Tropical Diseases Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Abbas Ahmadi ahmadi22@gmail.con 100319475328460021775 100319475328460021775 No Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Seyed mahmood Seyed khorrami khorami@yahoo.com 100319475328460021776 100319475328460021776 No Department of Medical Virology, Faculty of Medical Sciences, Tarbiat Modares University (TMU), Tehran, Iran Akran Sadat Ahmadi ahmadi25@yahoo.com 100319475328460021777 100319475328460021777 No Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Mohsen Akhondi-Meybodi shayesteh2009@yahoo.co.uk 100319475328460021778 100319475328460021778 Yes Department of Gastroenterology, School of Medicine, Shahid Sadoughi University of Medical Sciences,Yazd, Iran