en Infectious Diseases Infectious Diseases Original Article Original Article <span style="font-size:14px;"><span style="font-family:times new roman;"><span style="color:#0000FF;"><strong><em>Background</em></strong><em>:</em></span> Carbapenem resistance in <em>Acinetobacter baumannii </em>has become a major concern for treating physicians. The aim of this study was to investigate the prevalence of metallo &beta;-lactamase (MBL) genes (<em>bla _VIM , and bla_IMP</em>) among isolated multidrug-resistant <em>A. baumannii</em> .<br> <span style="color:#0000FF;"><strong><em>Methods</em></strong><em>:</em> </span>Fifty non-repetitive carbapenem-resistant <em>A. baumannii</em> isolates were collected. Antibiotic susceptibility was performed by disk diffusion method. MICs were determined by E test method. The resistant strains were tested for the production of carbapenemases by the Modified Hodge Test (MHT) followed by EDTA-disk synergy test was performed for metallo-&beta;-lactamases (MBL) phenotypic detection. Detection of <em>bla _VIM</em> , and <em>bla_IMP</em> was performed by PCR followed by sequencing.<br> <span style="color:#0000FF;"><strong><em>Results: </em></strong></span>All isolates had a multidrug resistant profile, and were all resistant to all antibiotics including the carbapenems but remained susceptible to colistin. Among these isolates, Carbapenemase production was confirmed by the Modified Hodge test for 42 (84%) isolates. Phenotypic method showed the production of MBL in 15 (30%) isolates. PCR techniques revealed that out of 50 isolates, 13 (26%) were positive for <em>bla_VIM</em> and all were negative for <em>bla_IMP.</em><br> <span style="color:#0000FF;"><strong><em>Conclusion</em></strong><em>:</em></span> Our study concludes that the high prevalence of carbapenem resistant <em>Acinetobacter </em>species with MBL production is one of the main concerns in our country and this situation needs strict infection control measures.</span></span> Acinetobacter baumannii, Carbapenem Resistance,Metallo-beta-Lactamases,Modified Hodge Test 171 176 http://caspjim.com/browse.php?a_code=A-10-26-7&slc_lang=en&sid=1 Zahra Moulana manigeh1345@gmail.com 100319475328460021752 100319475328460021752 No Infectious Diseases and Tropical Medicine Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran Arefeh Babazadeh Drbabazadeh.a@yahoo.com 100319475328460021753 100319475328460021753 No Infectious Diseases and Tropical Medicine Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, I.R. Iran Zohreh Eslamdost Drsoheil.d@gmail.com 100319475328460021754 100319475328460021754 No Department of Microbiology, Yahyanejad Hospital, Babol University of Medical Sciences, Babol, Iran Mehran Shokri Mshokri2015@yahoo.com 100319475328460021755 100319475328460021755 Yes Infectious Diseases and Tropical Medicine Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, I.R. Iran Soheil Ebrahimpour Drsoheil1503@yahoo.com 100319475328460021756 100319475328460021756 No Infectious Diseases and Tropical Medicine Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, I.R. Iran